Super-Enhancer-Associated Transcription Factors Maintain Transcriptional Regulation in Mature Podocytes.

ABSTRACT:

Background

Transcriptional programs control cell fate, and identifying their components is critical for understanding diseases caused by cell lesion, such as podocytopathy. Although many transcription factors (TFs) are necessary for cell-state maintenance in glomeruli, their roles in transcriptional regulation are not well understood.

Methods

The distribution of H3K27ac histones in human glomerulus cells was analyzed to identify superenhancer-associated TFs, and ChIP-seq and transcriptomics were performed to elucidate the regulatory roles of the TFs. Transgenic animal models of disease were further investigated to confirm the roles of specific TFs in podocyte maintenance.

Results

Superenhancer distribution revealed a group of potential TFs in core regulatory circuits

SUBMITTER: Yang J 

PROVIDER: S-EPMC8259645 | biostudies-literature | 2021 Jun

REPOSITORIES: biostudies-literature