Mechanical characterization of base analogue modified nucleic acids by force spectroscopy.
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ABSTRACT:
SUBMITTER: Sundar Rajan V
PROVIDER: S-EPMC8261857 | biostudies-literature | 2021 Jul
REPOSITORIES: biostudies-literature
Publications
Mechanical characterization of base analogue modified nucleic acids by force spectroscopy.
Sundar Rajan Vinoth V Viader-Godoy Xavier X Lin Yii-Lih YL Dutta Uttama U Ritort Felix F Westerlund Fredrik F Wilhelmsson L Marcus LM
Physical chemistry chemical physics : PCCP 20210701 26
We use mechanical unfolding of single DNA hairpins with modified bases to accurately assess intra- and intermolecular forces in nucleic acids. As expected, the modification stabilizes the hybridized hairpin, but we also observe intriguing stacking interactions in the unfolded hairpin. Our study highlights the benefit of using base-modified nucleic acids in force-spectroscopy. ...[more]
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