Translational Attenuation Mechanism of ErmB Induction by Erythromycin Is Dependent on Two Leader Peptides.

ABSTRACT: Ribosome stalling on ermBL at the tenth codon (Asp) is believed to be a major mechanism of ermB induction by erythromycin (Ery). In this study, we demonstrated that the mechanism of ermB induction by Ery depends not only on ermBL expression but also on previously unreported ermBL2 expression. Introducing premature termination codons in ermBL, we proved that translation of the N-terminal region of ermBL is the key component for ermB induced by Ery, whereas translation of the C-terminal region of ermBL did not affect Ery-induced ermB. Mutation of the tenth codon (Asp10) of ermBL with other amino acids showed that the degree of induction in vivo was not completely consistent with the data from the in vitro<

SUBMITTER: Wang S 

PROVIDER: S-EPMC8274638 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature