RiboNT: A Noise-Tolerant Predictor of Open Reading Frames from Ribosome-Protected Footprints.

ABSTRACT: Ribo-seq, also known as ribosome profiling, refers to the sequencing of ribosome-protected mRNA fragments (RPFs). This technique has greatly advanced our understanding of translation and facilitated the identification of novel open reading frames (ORFs) within untranslated regions or non-coding sequences as well as the identification of non-canonical start codons. However, the widespread application of Ribo-seq has been hindered because obtaining periodic RPFs requires a highly optimized protocol, which may be difficult to achieve, particularly in non-model organisms. Furthermore, the periodic RPFs are too short (28 nt) for accurate mapping to polyploid genomes, but longer RPFs are usually produced with a compromise in periodicity. Here we present RiboNT, a noise-tolerant ORF predictor tha

SUBMITTER: Song B 

PROVIDER: S-EPMC8307163 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature