Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing.
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ABSTRACT:
SUBMITTER: Cheng S
PROVIDER: S-EPMC8313749 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
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Protocol for interfering peptide injection into adult mouse hippocampus and spatial memory testing.
Cheng Shanshan S Shen Yuqian Y Zhang Zi Chao ZC Han Junhai J
STAR protocols 20210717 3
Metabotropic glutamate receptor-dependent long-term depression (mGluR-LTD) occurs in diverse brain regions and contributes to the plasticity of behavior, learning, and memory. mGluR-LTD relies on rapid (in minutes) local protein synthesis. Here, we describe a detailed protocol for delivering an interfering peptide into the adult mouse hippocampus. The delivered peptide disrupts the interaction between polyglutamine binding protein 1 and eukaryotic elongation factor 2, resulting in impaired hippo ...[more]
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