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Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study.

ABSTRACT: Irreversible electroporation (IRE) is a local non-thermal ablative technique currently used to treat solid tumors. Here, we investigated the clinical potency and safety of IRE with an endoscope in the upper gastrointestinal tract. Pigs were electroporated with recently designed endoscopic IRE catheters in the esophagus, stomach, and duodenum. Two successive strategies were introduced to optimize the electrical energy for the digestive tract. First, each organ was electroporated and the energy upscaled to confirm the upper limit energy inducing improper tissue results, including bleeding and perforation. Excluding the unacceptable energy from the first step, consecutive electroporations were performed with stepwise reductions in energy to identify the energy that damaged each layer. Inceptive research into inappropriate electrical intensity contributed to extensive hemorrhage and bowel perforation for each tissue above a certain energy threshold. However, experiments performed below the precluded energy accompanying hematoxylin and eosin staining and terminal deoxynucleotidyl transferase dUTP nick-end labeling assays showed that damaged mucosal area and depth significantly decreased with decreased energy. Relevant histopathology showed infiltration of inflammatory cells with pyknotic nuclei at the electroporated lesion. This investigation demonstrated the possibility of endoscopic IRE in mucosal dysplasia or early malignant tumors of the hollow viscus.

SUBMITTER: Jeon HJ

PROVIDER: S-EPMC8319327 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study.

Jeon Han Jo HJ Choi Hyuk Soon HS Keum Bora B Bang Eun Joo EJ Lee Kang Won KW Kim Sang Hyun SH Yim Sun Young SY Lee Jae Min JM Kim Eun Sun ES Seo Yeon Seok YS Jeen Yoon Tae YT Lee Hong Sik HS Chun Hoon Jai HJ Kim Hong Bae HB Kim Jong Hyuk JH

Scientific reports 20210728 1

Irreversible electroporation (IRE) is a local non-thermal ablative technique currently used to treat solid tumors. Here, we investigated the clinical potency and safety of IRE with an endoscope in the upper gastrointestinal tract. Pigs were electroporated with recently designed endoscopic IRE catheters in the esophagus, stomach, and duodenum. Two successive strategies were introduced to optimize the electrical energy for the digestive tract. First, each organ was electroporated and the energy up ...[more]

PMID: 34321494

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Project description:ImportanceUpper gastrointestinal tract cancer, including esophageal and gastric cancers, in China accounts for 50% of the global burden. Endoscopic screening may be associated with a decreased incidence of and mortality from upper gastrointestinal tract cancer.ObjectiveTo evaluate the cost-effectiveness of endoscopic screening for esophageal and gastric cancers among people aged 40 to 69 years in areas of China where the risk of these cancers is high.Design, setting, and participantsFor this economic evaluation, a Markov model was constructed for initial screening at different ages from a health care system perspective, and 5 endoscopic screening strategies with different frequencies (once per lifetime and every 10 years, 5 years, 3 years, and 2 years) were evaluated. The study was conducted between January 1, 2019, and October 31, 2020. Model parameters were estimated based on this project, government documents, and published literature. For each initial screening age (40-44, 45-49, 50-54, 55-59, 60-64, and 65-69 years), a closed cohort of 100 000 participants was assumed to enter the model and follow the alternative strategies.Main outcomes and measuresCost-effectiveness was measured by calculating the incremental cost-effectiveness ratio (ICER), and the willingness-to-pay threshold was assumed to be 3 times the per capita gross domestic product in China (US $10 276). Univariate and probabilistic sensitivity analyses were conducted to assess the robustness of model findings.ResultsThe study included a hypothetical cohort of 100 000 individuals aged 40 to 69 years. All 5 screening strategies were associated with improved effectiveness by 1087 to 10 362 quality-adjusted life-years (QALYs) and increased costs by US $3 299 000 to $22 826 000 compared with no screening over a lifetime, leading to ICERs of US $1343 to $3035 per QALY. Screening at a higher frequency was associated with an increase in QALYs and costs; ICERs for higher frequency screening compared with the next-lower frequency screening were between US $1087 and $4511 per QALY. Screening every 2 years would be the most cost-effective strategy, with probabilities of 90% to 98% at 3 times the per capita gross domestic product of China. The model was the most sensitive to utility scores of esophageal cancer- or gastric cancer-related health states and compliance with screening.Conclusions and relevanceThe findings suggest that combined endoscopic screening for esophageal and gastric cancers may be cost-effective in areas of China where the risk of these cancers is high; screening every 2 years would be the optimal strategy. These data may be useful for development of policies targeting the prevention and control of upper gastrointestinal tract cancer in China.

Project description:BackgroundCapsule endoscopy for visualization of the entire gastrointestinal tract is a challenge. A second-generation colon capsule endoscopy system (CCE-2) performed well in the colon and small intestine, but its utility in the upper gastrointestinal duct is not clear. We evaluated the use of the CCE-2 in the visualization of the upper gastrointestinal tract.MethodsWe performed a retrospective study and further evaluated CCE-2 images using the typical landmarks of esophagus and stomach. The two imagers located at each end of the CCE-2 system were defined as imager1 (green) and imager2 (yellow). Two endoscopists read the images, and they were blinded to the other reader's results. All of the images from the two imagers were separately reviewed.ResultsImages from 127 subjects were analyzed. This study demonstrated the comprehensive visualization of 71.7% of esophageal landmarks and 89.8% of gastric landmarks using the CCE-2. The two CCE-2 imagers were not identical, and the lighter imager (imager2, yellow) was superior to the heavier imager (imager1, green) (78% vs. 33.1%) in the stomach. Compared with the use of one imager, the use of two imagers was superior (two-imager vs. imager1, 89.8% vs. 33.1%; two-imager vs. imager2, 89.8% vs. 78%) in the stomach. Two-imager combination analysis detected a total of 160 positive findings. In contrast, single-imager analysis with imager1 and imager2 detected 133 and 137 findings, respectively. Two-imager combination analysis provided 20.3% and 16.8% more findings than imager1 and imager2, respectively. The two imagers complemented each other to detect more lesions.ConclusionsThe CCE-2 system is feasible for use in the upper gastrointestinal tract and may be considered an optional tool for upper gastrointestinal imaging. This system may represent a good choice for complete gastrointestinal duct screening. Compared with the use of one imager, the two-imager combination provided improved upper gastrointestinal tract mucosal visualization.

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Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study.

Publications

Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study.

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