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Human embryonic stem cell-derived cardiomyocyte platform screens inhibitors of SARS-CoV-2 infection.

ABSTRACT: Patients with cardiovascular comorbidities are more susceptible to severe infection with SARS-CoV-2, known to directly cause pathological damage to cardiovascular tissue. We outline a screening platform using human embryonic stem cell-derived cardiomyocytes, confirmed to express the protein machinery critical for SARS-CoV-2 infection, and a SARS-CoV-2 spike-pseudotyped virus system. The method has allowed us to identify benztropine and DX600 as novel inhibitors of SARS-CoV-2 infection in a clinically relevant stem cell-derived cardiomyocyte line. Discovery of new medicines will be critical for protecting the heart in patients with SARS-CoV-2, and for individuals where vaccination is contraindicated.

SUBMITTER: Williams TL

PROVIDER: S-EPMC8322398 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature

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Human embryonic stem cell-derived cardiomyocyte platform screens inhibitors of SARS-CoV-2 infection.

Williams Thomas L TL Colzani Maria T MT Macrae Robyn G C RGC Robinson Emma L EL Bloor Stuart S Greenwood Edward J D EJD Zhan Jun Ru JR Strachan Gregory G Kuc Rhoda E RE Nyimanu Duuamene D Maguire Janet J JJ Lehner Paul J PJ Sinha Sanjay S Davenport Anthony P AP

Communications biology 20210729 1

Patients with cardiovascular comorbidities are more susceptible to severe infection with SARS-CoV-2, known to directly cause pathological damage to cardiovascular tissue. We outline a screening platform using human embryonic stem cell-derived cardiomyocytes, confirmed to express the protein machinery critical for SARS-CoV-2 infection, and a SARS-CoV-2 spike-pseudotyped virus system. The method has allowed us to identify benztropine and DX600 as novel inhibitors of SARS-CoV-2 infection in a clini ...[more]

PMID: 34326460

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