Next generation sequencing for diagnosis of central nervous system aspergillosis in liver transplant recipients.

ABSTRACT:

Background

Fungal encephalitis is uncommon and sometimes fatal in liver transplant (LT) recipients. Early diagnosis of central nervous system (CNS) fungal infections, especially aspergillosis, is difficult based on routine tests of cerebrospinal fluid (CSF) alone. Next-generation sequencing (NGS) as a new tool may help in this respect.

Methods

Shotgun metagenomics was used to detect pathogens in CSF of patients, who were clinically suspected of CNS infection. Sequencing was performed at BGIseq-50 platform (BGI, Shenzhen).

Results

NGS technique identified Aspergillus in CSF of 5 patients, who were suspected of CNS infection, although clinical symptoms of these patients varied dramatically. The resulting sequence reads corresponding to Aspergillus species

SUBMITTER: Chen F 

PROVIDER: S-EPMC8339870 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature