Removal of ²H-decoupling sidebands in ¹³CHD₂ ¹³C-CEST profiles.

ABSTRACT: A unique aspect of NMR is its capacity to provide integrated insight into both the structure and intrinsic dynamics of biomolecules. Chemical exchange phenomena that often serve as probes of dynamic processes in biological macromolecules can be quantitatively investigated with chemical exchange saturation transfer (CEST) experiments. ²H-decoupling sidebands, however, always occur in the profiles of ¹³CHD₂ ¹³C-CEST experiments when using the simple CW (continuous wave) method, which may obscure the detection of minor dips of excited states. Traditionally, these sidebands are manually eliminated from the profiles before data analysis by removing experimental points in the range of ²H-decoupling field strength ±50 Hz away from the major d

SUBMITTER: Xia Y 

PROVIDER: S-EPMC8342043 | biostudies-literature | 2021 Mar

REPOSITORIES: biostudies-literature