Azithromycin induces apoptosis in airway smooth muscle cells through mitochondrial pathway in a rat asthma model.

ABSTRACT:

Background

The proliferation of airway smooth muscle cells (ASMCs) is a key feature of airway remodeling in asthma. Azithromycin (AZM) has been shown to decrease bronchial hyperresponsiveness and airway inflammation in asthmatics; however, the role of AZM in ASMC proliferation remains unclear. Thus, we investigated the effect of AZM on ASMC proliferation in a rat model of experimental asthma.

Methods

We isolated ASMCs from rats sensitized and challenged by ovabulmin (OVA), and then treated with different concentrations of AZM. Cytotoxicity of ASMC was evaluated by Cell Counting Kit-8 (CCK-8) assay, morphological change was examined with laser confocal microscope after Annexin V/propidium iodide (PI) double staining, mitochondrial membrane potential was determined with JC-1 s

SUBMITTER: Wu L 

PROVIDER: S-EPMC8350718 | biostudies-literature | 2021 Jul

REPOSITORIES: biostudies-literature