Large-scale, three-dimensional tissue cytometry of the human kidney: a complete and accessible pipeline.

ABSTRACT: The advent of personalized medicine has driven the development of novel approaches for obtaining detailed cellular and molecular information from clinical tissue samples. Tissue cytometry is a promising new technique that can be used to enumerate and characterize each cell in a tissue and, unlike flow cytometry and other single-cell techniques, does so in the context of the intact tissue, preserving spatial information that is frequently crucial to understanding a cell's physiology, function, and behavior. However, the wide-scale adoption of tissue cytometry as a research tool has been limited by the fact that published examples utilize specialized techniques that are beyond the capabilities of most laboratories. Here we describe a complete and accessible pipeline, including methods of sam

SUBMITTER: Ferkowicz MJ 

PROVIDER: S-EPMC8363780 | biostudies-literature | 2021 May

REPOSITORIES: biostudies-literature