ProteaseGuru: A Tool for Protease Selection in Bottom-Up Proteomics.

ABSTRACT: Bottom-up proteomics is currently the dominant strategy for proteome analysis. It relies critically upon the use of a protease to digest proteins into peptides, which are then identified by liquid chromatography-mass spectrometry (LC-MS). The choice of protease(s) has a substantial impact upon the utility of the bottom-up results obtained. Protease selection determines the nature of the peptides produced, which in turn affects the ability to infer the presence and quantities of the parent proteins and post-translational modifications in the sample. We present here the software tool ProteaseGuru, which provides in silico digestions by candidate proteases, allowing evaluation of their utility for bottom-up proteomic experiments. This information is useful for both studies focused on a single

SUBMITTER: Miller RM 

PROVIDER: S-EPMC8366505 | biostudies-literature | 2021 Apr

REPOSITORIES: biostudies-literature