Molecular signaling of synthetic cannabinoids: Comparison of CB1 receptor and TRPV1 channel activation.

ABSTRACT: Recreational use of synthetic cannabinoids (SCs) is associated with desirable euphoric and relaxation effects as well as adverse effects including anxiety, agitation and psychosis. These SC-mediated actions represent a combination of potentiated cannabinoid receptor signaling and "off-target" receptor activity. The goal of this study was to compare the efficacy of various classes of SCs in stimulating CB₁ receptors and activating "off-target" transient receptor potential (TRP) channels. Cannabinoid-type 1 (CB₁) receptor activity was determined by measuring SC activation of G protein-gated inward rectifier K⁺ (GIRK) channels using a membrane potential-sensitive fluorescent dye assay. SC opening of vanilloid type-1 (TRPV1) channels was measured by recording i

SUBMITTER: Andersen HK 

PROVIDER: S-EPMC8374946 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature