Designer membraneless organelles sequester native factors for control of cell behavior.

ABSTRACT: Subcellular compartmentalization of macromolecules increases flux and prevents inhibitory interactions to control biochemical reactions. Inspired by this functionality, we sought to build designer compartments that function as hubs to regulate the flow of information through cellular control systems. We report a synthetic membraneless organelle platform to control endogenous cellular activities through sequestration and insulation of native proteins. We engineer and express a disordered protein scaffold to assemble micron-size condensates and recruit endogenous clients via genomic tagging with high-affinity dimerization motifs. By relocalizing up to 90% of targeted enzymes to synthetic condensates, we efficiently control cellular behaviors, including proliferation, division and cytoskeleta

SUBMITTER: Garabedian MV 

PROVIDER: S-EPMC8387445 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature