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A Method of Solubilizing and Concentrating Astaxanthin and Other Carotenoids.

ABSTRACT: The valuable marine carotenoid, astaxanthin, is used in supplements, medicines and cosmetics. In this study, crustacyanin, an astaxanthin-binding protein, was used to solubilize and concentrate astaxanthin. The recombinant crustacyanin of European lobster spontaneously formed an inclusion body when it was over-expressed in Escherichia coli. In this study, fusing the NusA-tag to the crustacyanin subunits made it possible to express in a soluble fraction and solubilize astaxanthin in aqueous solution. By cutting off the NusA-tag, the crustacyanin subunits generated the pure insoluble form, and captured and concentrated astaxanthin. Overall, the attaching and releasing NusA-tag method has the potential to supply solubilized carotenoids in aqueous solution and concentrated carotenoids, respectively.

SUBMITTER: Hara KY

PROVIDER: S-EPMC8399374 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature

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A Method of Solubilizing and Concentrating Astaxanthin and Other Carotenoids.

Hara Kiyotaka Y KY Yagi Shuwa S Hirono-Hara Yoko Y Kikukawa Hiroshi H

Marine drugs 20210816 8

The valuable marine carotenoid, astaxanthin, is used in supplements, medicines and cosmetics. In this study, crustacyanin, an astaxanthin-binding protein, was used to solubilize and concentrate astaxanthin. The recombinant crustacyanin of European lobster spontaneously formed an inclusion body when it was over-expressed in <i>Escherichia coli</i>. In this study, fusing the NusA-tag to the crustacyanin subunits made it possible to express in a soluble fraction and solubilize astaxanthin in aqueou ...[more]

PMID: 34436301

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