Glycoprotein analysis using lectin microcolumns and capillary electrophoresis: Characterization of alpha₁-acid glycoprotein by combined separation methods.

ABSTRACT: Separations based on combinations of 2.1 mm I.D. high-performance affinity microcolumns and capillary electrophoresis were developed and used to characterize the glycoforms of an intact glycoprotein. Human alpha₁-acid glycoprotein (AGP) was used as a model analyte due to its heterogeneous glycosylation resulting from variations in its degree of branching, fucosylation, and number of sialic acids. Three separation formats were examined based on microcolumns that contained the lectins concanavalin A (Con A) or Aleuria aurantia lectin (AAL). These microcolumns were used with one another or in combination with capillary electrophoresis. N-Glycan analysis of the non-retained and retained AGP fractions was carried out by using PNGase F digestion and nanoflow electrospray ionization ma

SUBMITTER: Zhang C 

PROVIDER: S-EPMC8403148 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature