A new Trypanosoma cruzi genotyping method enables high resolution evolutionary analyses.

ABSTRACT:

Background

Trypanosoma cruzi is an important human pathogen in Latin America with nearly seven million people infected. It has a large degree of genetic diversity, classified into six discrete typing units (DTUs), which probably influences its physiological behavior and clinical manifestations. Several genotyping methods are available, with distinct performance on easiness, cost, resolution and applicability; no method excels in all parameters.

Objectives and methods

To devise a molecular method for T. cruzi genotyping, based on polymerase chain reaction (PCR) amplification of a single target with multiple copies in the nuclear genome by large scale sequencing. We have applied this method to 29 T. cruzi isolates, comprising all described DTUs.

Findings

We were able to

SUBMITTER: Probst CM 

PROVIDER: S-EPMC8405150 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature