Dataset Information

Downregulated expression of S₂-RNase attenuates self-incompatibility in "Guiyou No. 1" pummelo.

ABSTRACT: Self-incompatibility (SI) substantially restricts the yield and quality of citrus. Therefore, breeding and analyzing self-compatible germplasm is of great theoretical and practical significance for citrus. Here, we focus on the mechanism of a self-compatibility mutation in 'Guiyou No. 1' pummelo (Citrus maxima), which is a spontaneous mutant of 'Shatian' pummelo (Citrus maxima, self-incompatibility). The rate of fruit set and the growth of pollen tubes in the pistil confirmed that a spontaneous mutation in the pistil is responsible for the self-compatibility of 'Guiyou No. 1'. Segregation ratios of the S genotype in F₁ progeny, expression analysis, and western blotting validated that the reduced levels of S₂-RNase mRNA contribute to the loss of SI in 'Guiyou No. 1'. Furthermore, we report a phased assembly of the 'Guiyou No. 1' pummelo genome and obtained two complete and well-annotated S haplotypes. Coupled with an analysis of SV variations, methylation levels, and gene expression, we identified a candidate gene (CgHB40), that may influence the regulation of the S₂-RNase promoter. Our data provide evidence that a mutation that affects the pistil led to the loss of SI in 'Guiyou No. 1' by influencing a poorly understood mechanism that affects transcriptional regulation. This work significantly advances our understanding of the genetic basis of the SI system in citrus and provides information on the regulation of S-RNase genes.

SUBMITTER: Hu J

PROVIDER: S-EPMC8408199 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Downregulated expression of S<sub>2</sub>-RNase attenuates self-incompatibility in "Guiyou No. 1" pummelo.

Hu Jianbing J Xu Qiang Q Liu Chenchen C Liu Binghao B Deng Chongling C Chen Chuanwu C Wei Zhuangmin Z Ahmad Muhammad Husnain MH Peng Kang K Wen Hao H Chen Xiangling X Chen Peng P Larkin Robert M RM Ye Junli J Deng Xiuxin X Chai Lijun L

Horticulture research 20210901 1

Self-incompatibility (SI) substantially restricts the yield and quality of citrus. Therefore, breeding and analyzing self-compatible germplasm is of great theoretical and practical significance for citrus. Here, we focus on the mechanism of a self-compatibility mutation in 'Guiyou No. 1' pummelo (Citrus maxima), which is a spontaneous mutant of 'Shatian' pummelo (Citrus maxima, self-incompatibility). The rate of fruit set and the growth of pollen tubes in the pistil confirmed that a spontaneous ...[more]

PMID: 34465762

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Project description:BACKGROUND:Fabaceae species are important in agronomy and livestock nourishment. They have a long breeding history, and most cultivars have lost self-incompatibility (SI), a genetic barrier to self-fertilization. Nevertheless, to improve legume crop breeding, crosses with wild SI relatives of the cultivated varieties are often performed. Therefore, it is fundamental to characterize Fabaceae SI system(s). We address the hypothesis of Fabaceae gametophytic (G)SI being RNase based, by recruiting the same S-RNase lineage gene of Rosaceae, Solanaceae or Plantaginaceae SI species. RESULTS:We first identify SSK1 like genes (described only in species having RNase based GSI), in the Trifolium pratense, Medicago truncatula, Cicer arietinum, Glycine max, and Lupinus angustifolius genomes. Then, we characterize the S-lineage T2-RNase genes in these genomes. In T. pratense, M. truncatula, and C. arietinum we identify S-RNase lineage genes that in phylogenetic analyses cluster with Pyrinae S-RNases. In M. truncatula and C. arietinum genomes, where large scaffolds are available, these sequences are surrounded by F-box genes that in phylogenetic analyses also cluster with S-pollen genes. In T. pratense the S-RNase lineage genes show, however, expression in tissues not involved in GSI. Moreover, levels of diversity are lower than those observed for other S-RNase genes. The M. truncatula and C. arietinum S-RNase and S-pollen like genes phylogenetically related to Pyrinae S-genes, are also expressed in tissues other than those involved in GSI. To address if other T2-RNases could be determining Fabaceae GSI, here we obtained a style with stigma transcriptome of Cytisus striatus, a species that shows significant difference on the percentage of pollen growth in self and cross-pollinations. Expression and polymorphism analyses of the C. striatus S-RNase like genes revealed that none of these genes, is the S-pistil gene. CONCLUSION:We find no evidence for Fabaceae GSI being determined by Rosaceae, Solanaceae, and Plantaginaceae S-RNase lineage genes. There is no evidence that T2-RNase lineage genes could be determining GSI in C. striatus. Therefore, to characterize the Fabaceae S-pistil gene(s), expression analyses, levels of diversity, and segregation analyses in controlled crosses are needed for those genes showing high expression levels in the tissues where GSI occurs.

Dataset Information

Downregulated expression of S₂-RNase attenuates self-incompatibility in "Guiyou No. 1" pummelo.

Publications

Downregulated expression of S<sub>2</sub>-RNase attenuates self-incompatibility in "Guiyou No. 1" pummelo.

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Dataset Information

Downregulated expression of S2-RNase attenuates self-incompatibility in "Guiyou No. 1" pummelo.

Publications

Downregulated expression of S<sub>2</sub>-RNase attenuates self-incompatibility in "Guiyou No. 1" pummelo.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets

Downregulated expression of S₂-RNase attenuates self-incompatibility in "Guiyou No. 1" pummelo.