Downregulated expression of S₂-RNase attenuates self-incompatibility in "Guiyou No. 1" pummelo.

ABSTRACT: Self-incompatibility (SI) substantially restricts the yield and quality of citrus. Therefore, breeding and analyzing self-compatible germplasm is of great theoretical and practical significance for citrus. Here, we focus on the mechanism of a self-compatibility mutation in 'Guiyou No. 1' pummelo (Citrus maxima), which is a spontaneous mutant of 'Shatian' pummelo (Citrus maxima, self-incompatibility). The rate of fruit set and the growth of pollen tubes in the pistil confirmed that a spontaneous mutation in the pistil is responsible for the self-compatibility of 'Guiyou No. 1'. Segregation ratios of the S genotype in F₁ progeny, expression analysis, and western blotting validated that the reduced levels of S₂-RNase mRNA contribute to the loss of SI in 'Guiyou No. 1'. F

SUBMITTER: Hu J 

PROVIDER: S-EPMC8408199 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature