Study on mechanism of low bioavailability of black tea theaflavins by using Caco-2 cell monolayer.

ABSTRACT: This study aimed to clarify the bioavailability mechanism of theaflavins by using the Caco-2 monolayer in vitro model. Prior to the transport of theaflavin (TF), theaflavin-3-gallate (TF3G), theaflavin-3'-gallate (TF3'G), and theaflavin-3, 3'-digallate (TFDG), we found the cytotoxicity of theaflavins was in the order of TF3'G > TFDG > TF3G > TF, suggesting the galloyl moiety enhances the cytotoxicity of theaflavins. Meantime, the galloyl moiety made theaflavins unstable, with the stability in the order of TF > TFDG > TF3G/TF3'G. Four theaflavins showed poor bioavailability with the P_app values ranging from 0.44 × 10^-7 to 3.64 × 10^-7 cm/s in the absorptive transport. All the theaflavins showed an efflux ratio of over 1.24. And it is further con

SUBMITTER: Qu F 

PROVIDER: S-EPMC8409943 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature