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Integrin-α_V-mediated activation of TGF-β regulates anti-tumour CD8 T cell immunity and response to PD-1 blockade.

ABSTRACT: TGF-β is secreted in the tumour microenvironment in a latent, inactive form bound to latency associated protein and activated by the integrin α_V subunit. The activation of latent TGF-β by cancer-cell-expressed α_V re-shapes the tumour microenvironment, and this could affect patient responses to PD-1-targeting therapy. Here we show, using multiplex immunofluorescence staining in cohorts of anti-PD-1 and anti-PD-L1-treated lung cancer patients, that decreased expression of cancer cell α_V is associated with improved immunotherapy-related, progression-free survival, as well as with an increased density of CD8⁺CD103⁺ tumour-infiltrating lymphocytes. Mechanistically, tumour α_V regulates CD8 T cell recruitment, induces CD103 expression on activated CD8⁺ T cells and promotes their differentiation to granzyme B-producing CD103⁺CD69⁺ resident memory T cells via autocrine TGF-β signalling. Thus, our work provides the underlying principle of targeting cancer cell α_V for more efficient PD-1 checkpoint blockade therapy.

SUBMITTER: Malenica I

PROVIDER: S-EPMC8410945 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Integrin-αV-mediated activation of TGF-β regulates anti-tumour CD8 T cell immunity and response to PD-1 blockade.

Malenica Ines I Adam Julien J Corgnac Stéphanie S Mezquita Laura L Auclin Edouard E Damei Isabelle I Grynszpan Laetitia L Gros Gwendoline G de Montpréville Vincent V Planchard David D Théret Nathalie N Besse Benjamin B Mami-Chouaib Fathia F

Nature communications 20210901 1

TGF-β is secreted in the tumour microenvironment in a latent, inactive form bound to latency associated protein and activated by the integrin αV subunit. The activation of latent TGF-β by cancer-cell-expressed αV re-shapes the tumour microenvironment, and this could affect patient responses to PD-1-targeting therapy. Here we show, using multiplex immunofluorescence staining in cohorts of anti-PD-1 and anti-PD-L1-treated lung cancer patients, that decreased expression of can ...[more]

PMID: 34471106

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Project description:BACKGROUNDHead and neck squamous cell carcinoma not associated with HPV (HPV-unrelated HNSCC) is associated with a high rate of recurrence and poor survival.METHODSWe conducted a clinical trial in 14 patients with newly diagnosed HPV-unrelated HNSCC to evaluate the safety and efficacy of neoadjuvant bintrafusp alfa, a bifunctional fusion protein that blocks programmed death ligand 1 (PD-L1) and neutralizes TGF-β.RESULTSBintrafusp alfa was well tolerated, and no treatment-associated surgical delays or complications occurred. Objective pathologic responses (PRs) were observed, and 12 of the 14 (86%) patients were alive and disease free at 1 year. Alterations in Treg infiltration and spatial distribution relative to proliferating CD8+ T cells indicated a reversal of Treg immunosuppression in the primary tumor. Detection of neoepitope-specific tumor T cell responses, but not virus-specific responses, correlated with the development of a PR. Detection of neoepitope-specific responses and PRs in tumors was not correlated with genomic features or tumor antigenicity but was associated with reduced pretreatment myeloid cell tumor infiltration. These results indicate that dual PD-L1 and TGF-β blockade can safely enhance tumor antigen-specific immunity and highlight the feasibility of multimechanism neoadjuvant immunotherapy for patients with HPV-unrelated HNSCC.CONCLUSIONOur studies provide insight into the ability of neoadjuvant immunotherapy to induce polyclonal neoadjuvant-specific T cell responses in tumors and suggest that features of the tumor microenvironment, such as myeloid cell infiltration, may be a major determinant of enhanced antitumor immunity following such treatment.TRIAL REGISTRATIONClinicalTrials.gov NCT04247282.FUNDINGThis work was funded by the Center for Cancer Research, the NCI, and the Intramural Research Program of the NIDCD, NIH. Bintrafusp alfa was provided by the health care business of Merck KGaA (Darmstadt, Germany), through a Cooperative Research and Development Agreement with the NCI. Additional funding was provided by ImmunityBio through a Cooperative Research and Development Agreement with the NIDCD.

Dataset Information

Integrin-α_V-mediated activation of TGF-β regulates anti-tumour CD8 T cell immunity and response to PD-1 blockade.

Publications

Integrin-α<sub>V</sub>-mediated activation of TGF-β regulates anti-tumour CD8 T cell immunity and response to PD-1 blockade.

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