Intraspinal injection of adeno-associated viruses into the adult mouse spinal cord.
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ABSTRACT:
SUBMITTER: Chaterji S
PROVIDER: S-EPMC8414904 | biostudies-literature | 2021 Sep
REPOSITORIES: biostudies-literature
Publications
Intraspinal injection of adeno-associated viruses into the adult mouse spinal cord.
Chaterji Shrivas S Barik Arnab A Sathyamurthy Anupama A
STAR protocols 20210901 3
Genetic dissection of neural circuits has been accelerated by recent advances in viral-based vectors. This protocol describes an effective approach to performing intraspinal injections of adeno-associated viruses, which can be used to label, manipulate, and monitor spinal and supraspinal neurons. By avoiding invasive laminectomies and restrictive spinal-clamping and by adopting injectable anaesthetics and tough quartz glass micropipettes, our protocol presents a time-saving and efficient approac ...[more]
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