METTL3-mediated m6A methylation of ASPM drives hepatocellular carcinoma cells growth and metastasis.

ABSTRACT:

Background

Abnormal spindle-like microcephaly (ASPM) has been proved to participate in tumor progression. However, the underlying mechanism of ASPM in liver hepatocellular carcinoma (LIHC) remains elusive.

Methods

The mRNA and protein expression were determined using Western blot and qRT-PCR, and the capacities of cells proliferation, migration, and invasion were evaluated by CCK-8, colony formation, wound healing, and transwell. MeRIP was performed to validate the interaction between ASPM and methyltransferase-like 3 (METTL3).

Results

Herein, we found that ASPM was significantly upregulated in LIHC, and the high expression of ASPM was associated with poor LIHC prognosis. Furthermore, ASPM knockdown could suppress LIHC cells proliferation, migration, and invasion, whi

SUBMITTER: Wang A 

PROVIDER: S-EPMC8418466 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature