Immune Checkpoint Inhibitors Regulate K⁺ Channel Activity in Cytotoxic T Lymphocytes of Head and Neck Cancer Patients.

ABSTRACT: Programmed death receptor-1 (PD-1) and its ligand (PD-L1) interaction negatively regulates T cell function in head and neck squamous cell carcinoma (HNSCC). Overexpression of PD-1 reduces intracellular Ca²⁺ fluxes, and thereby T cell effector functions. In HNSCC patients, PD-1 blockade increases KCa3.1 and Kv1.3 activity along with Ca²⁺ signaling and mobility in CD8⁺ peripheral blood T cells (PBTs). The mechanism by which PD-L1/PD-1 interaction regulates ion channel function is not known. We investigated the effects of blocking PD-1 and PD-L1 on ion channel functions and intracellular Ca²⁺ signaling in CD8⁺ PBTs of HNSCC patients and healthy donors (HDs) using single-cell electrophysiology and live microscopy. Anti-PD-1 and anti-PD-L1

SUBMITTER: Gawali VS 

PROVIDER: S-EPMC8429813 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature