Stationary-phase Mutagenesis Soft-agar Overlay Assays in Bacillus subtilis.

ABSTRACT: Elucidating how a population of non-growing bacteria generates mutations improves our understanding of phenomena like antibiotic resistance, bacterial pathogenesis, genetic diversity and evolution. To evaluate mutations that occur in nutritionally stressed non-growing bacteria, we have employed the strain B. subtilis YB955, which measures the reversions rates to the chromosomal auxotrophies hisC952, metB5 and leuC427 (Sung and Yasbin, 2002). This gain-of-function system has successfully allowed establishing the role played by repair systems and transcriptional factors in stress-associated mutagenesis (SPM) (Barajas- Ornelas et al., 2014 ; Gómez- Marroquín et al., 2016 ). In a recent study (Castro- Cerritos et al., 2017 ), it was found that Ribonucl

SUBMITTER: Castro-Cerritos KV 

PROVIDER: S-EPMC8438431 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature