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Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs.

ABSTRACT: Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABA_A antibodies allowed for site-specific membrane potential measurements from postsynaptic sites.

SUBMITTER: Grupi A

PROVIDER: S-EPMC8448295 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Point-localized, site-specific membrane potential optical recording by single fluorescent nanodiscs.

Grupi Asaf A Shapira Zehavit Z Yudovich Shimon S Degani-Katzav Nurit N Weiss Shimon S

Biophysical reports 20210908 1

Nanodisc technology was implemented as a platform for voltage nanosensors. A fluorescence (Förster) resonance energy transfer (FRET)- based voltage-sensing scheme employing fluorescent nanodiscs and the hydrophobic ion dipicrylamine was developed and utilized to optically record membrane potentials on the single-nanodisc level. Ensemble and single-nanosensor recordings were demonstrated for HEK293 cells and primary cortical neuron cells. Conjugation of nanodiscs to anti-GABA<sub>A</sub> antibodi ...[more]

PMID: 34568861

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