HIV reprograms host m⁶Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1.

ABSTRACT: N⁶,2'-O-dimethyladenosine (m⁶Am) is an abundant RNA modification located adjacent to the 5'-end of the mRNA 7-methylguanosine (m⁷G) cap structure. m⁶A methylation on 2'-O-methylated A at the 5'-ends of mRNAs is catalyzed by the methyltransferase Phosphorylated CTD Interacting Factor 1 (PCIF1). The role of m⁶Am and the function of PCIF1 in regulating host-pathogens interactions are unknown. Here, we investigate the dynamics and reprogramming of the host m⁶Am RNA methylome during HIV infection. We show that HIV infection induces a dramatic decrease in m⁶Am of cellular mRNAs. By using PCIF1 depleted T cells, we identify 2237 m⁶Am genes and 854 are affected by HIV infection. Strikingly, we find that PCIF1 m

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC8452764 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature