Comparing periodontitis biomarkers in saliva, oral rinse and gingival crevicular fluid: A pilot study.

ABSTRACT:

Aim

To explore the feasibility of screening for periodontitis by measuring biomarkers, namely total proteolytic activity (TPA), matrix metalloproteinase (MMP)-8, chitinase, lysozyme or their combination, in saliva, oral rinse and gingival crevicular fluid (GCF).

Material and methods

Subjects were recruited among healthy/gingivitis individuals and untreated periodontitis patients in Academic Centre for Dentistry Amsterdam (ACTA). All participants donated samples of unstimulated whole saliva, oral rinse and GCF. The protein concentrations and MMP-8 levels were determined by ELISA. Enzymatic activities were measured using appropriate fluorogenic substrates.

Results

In oral rinse samples, periodontitis patients (n = 19) exhibited significantly higher concentrations of MMP

SUBMITTER: Katsiki P 

PROVIDER: S-EPMC8453974 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature