Selective nuclear export of mRNAs is promoted by DRBD18 in Trypanosoma brucei.

ABSTRACT: Kinetoplastids, including Trypanosoma brucei, control gene expression primarily at the posttranscriptional level. Nuclear mRNA export is an important, but understudied, step in this process. The general heterodimeric export factors, Mex67/Mtr2, function in the export of mRNAs and tRNAs in T. brucei, but RNA binding proteins (RBPs) that regulate export processes by controlling the dynamics of Mex67/Mtr2 ribonucleoprotein formation or transport have not been identified. Here, we report that DRBD18, an essential and abundant T. brucei RBP, associates with Mex67/Mtr2 in vivo, likely through its direct interaction with Mtr2. DRBD18 downregulation results in partial accumulation of poly(A)⁺ mRNA in the nucleus, but has no effect on the localization of intron-containing or mature tRNAs

SUBMITTER: Mishra A 

PROVIDER: S-EPMC8460611 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature