Prospective pharmacological methodology for establishing and evaluating anti-cancer drug resistant cell lines.

ABSTRACT:

Background

Cell lines are often used to assess the resistance of anticancer drugs when in vivo analysis is not possible. However, the process for establishing anti-cancer drug resistance in cell cultures in vitro and the subsequent method of then evaluating resistance are not clearly established. Traditionally, the IC₅₀ is the most commonly used indicator of resistance evaluation but it cannot represent the effectiveness of anti-cancer drugs in a clinical setting and lacks reliability because it is heavily affected by the cell doubling time. Hence, new indicators that can evaluate anti-cancer drug resistance are needed.

Methods

A novel resistance evaluation methodology was validated in this present study by establishing sunitinib resistance in renal cell carcinoma

SUBMITTER: Yu H 

PROVIDER: S-EPMC8464141 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature