IRF4 ablation in B cells abrogates allogeneic B cell responses and prevents chronic transplant rejection.

ABSTRACT:

Backgound

B cells contribute to chronic transplant rejection by producing donor-specific antibodies and promoting T cell response, but how these processes are regulated at the transcriptional level remains unclear. Herein, we investigate the role of transcription factor interferon regulatory factor 4 (IRF4) in controlling B cell response during chronic transplant rejection.

Methods

We generated the Irf4^gfp reporter mice to determine IRF4 expression in B cell lineage. We then used mice with B cell-specific IRF4 deletion to define the role of IRF4 in B cell response after NP-KLH immunization or allogeneic heart transplantation. In particular, graft survival and histology, as well as B and T cell responses, were evaluated after transplantation.

Results

IRF4 is

SUBMITTER: Wang G 

PROVIDER: S-EPMC8464533 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature