Metabolite Ratios as Quality Indicators for Pre-Analytical Variation in Serum and EDTA Plasma.

ABSTRACT: In clinical diagnostics and research, blood samples are one of the most frequently used materials. Nevertheless, exploring the chemical composition of human plasma and serum is challenging due to the highly dynamic influence of pre-analytical variation. A prominent example is the variability in pre-centrifugation delay (time-to-centrifugation; TTC). Quality indicators (QI) reflecting sample TTC are of utmost importance in assessing sample history and resulting sample quality, which is essential for accurate diagnostics and conclusive, reproducible research. In the present study, we subjected human blood to varying TTCs at room temperature prior to processing for plasma or serum preparation. Potential sample QIs were identified by Ultra high pressure liquid chromatography tandem mass spectr

SUBMITTER: Heiling S 

PROVIDER: S-EPMC8465943 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature