Subgenome Discrimination in Brassica and Raphanus Allopolyploids Using Microsatellites.

ABSTRACT: Intergeneric crosses between Brassica species and Raphanus sativus have produced crops with prominent shoot and root systems of Brassica and R. sativus, respectively. It is necessary to discriminate donor genomes when studying cytogenetic stability in distant crosses to identify homologous chromosome pairing, and microsatellite repeats have been used to discriminate subgenomes in allopolyploids. To identify genome-specific microsatellites, we explored the microsatellite content in three Brassica species (B. rapa, AA, B. oleracea, CC, and B. nigra, BB) and R. sativus (RR) genomes, and validated their genome specificity by fluorescence in situ hybridization. We identified three microsatellites showing A, C, and B/R genome specifi

SUBMITTER: Campomayor NB 

PROVIDER: S-EPMC8466703 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature