Dataset Information

Discovery of Genes that Modulate Flavivirus Replication in an Interferon-Dependent Manner.

ABSTRACT: Establishment of the interferon (IFN)-mediated antiviral state provides a crucial initial line of defense against viral infection. Numerous genes that contribute to this antiviral state remain to be identified. Using a loss-of-function strategy, we screened an original library of 1156 siRNAs targeting 386 individual curated human genes in stimulated microglial cells infected with Zika virus (ZIKV), an emerging RNA virus that belongs to the flavivirus genus. The screen recovered twenty-one potential host proteins that modulate ZIKV replication in an IFN-dependent manner, including the previously known IFITM3 and LY6E. Further characterization contributed to delineate the spectrum of action of these genes towards other pathogenic RNA viruses, including Hepatitis C virus and SARS-CoV-2. Our data revealed that APOL3 acts as a proviral factor for ZIKV and several other related and unrelated RNA viruses. In addition, we showed that MTA2, a chromatin remodeling factor, possesses potent flavivirus-specific antiviral functions induced by IFN. Our work identified previously unrecognized genes that modulate the replication of RNA viruses in an IFN-dependent manner, opening new perspectives to target weakness points in the life cycle of these viruses.

SUBMITTER: Lesage S

PROVIDER: S-EPMC8480147 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature

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Discovery of Genes that Modulate Flavivirus Replication in an Interferon-Dependent Manner.

Lesage Sarah S Chazal Maxime M Beauclair Guillaume G Batalie Damien D Cerboni Silvia S Couderc Elodie E Lescure Aurianne A Del Nery Elaine E Tangy Frédéric F Martin Annette A Manel Nicolas N Jouvenet Nolwenn N

Journal of molecular biology 20210929 6

Establishment of the interferon (IFN)-mediated antiviral state provides a crucial initial line of defense against viral infection. Numerous genes that contribute to this antiviral state remain to be identified. Using a loss-of-function strategy, we screened an original library of 1156 siRNAs targeting 386 individual curated human genes in stimulated microglial cells infected with Zika virus (ZIKV), an emerging RNA virus that belongs to the flavivirus genus. The screen recovered twenty-one potent ...[more]

PMID: 34599939

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Dataset Information

Discovery of Genes that Modulate Flavivirus Replication in an Interferon-Dependent Manner.

Publications

Discovery of Genes that Modulate Flavivirus Replication in an Interferon-Dependent Manner.

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