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Involvement of RNA granule proteins in meiotic silencing by unpaired DNA.

ABSTRACT: In Neurospora crassa, expression from an unpaired gene is suppressed by a mechanism known as meiotic silencing by unpaired DNA (MSUD). MSUD utilizes common RNA interference (RNAi) factors to silence target mRNAs. Here, we report that Neurospora CAR-1 and CGH-1, homologs of two Caenorhabditis elegans RNA granule components, are involved in MSUD. These fungal proteins are found in the perinuclear region and P-bodies, much like their worm counterparts. They interact with components of the meiotic silencing complex (MSC), including the SMS-2 Argonaute. This is the first time MSUD has been linked to RNA granule proteins.

SUBMITTER: Xiao H

PROVIDER: S-EPMC8482848 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature

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Involvement of RNA granule proteins in meiotic silencing by unpaired DNA.

Xiao Hua H Vierling Michael M MM Kennedy Rana F RF Boone Erin C EC Decker Logan M LM Sy Victor T VT Haynes Jackson B JB Williams Michelle A MA Shiu Patrick K T PKT

G3 (Bethesda, Md.) 20210901 10

In Neurospora crassa, expression from an unpaired gene is suppressed by a mechanism known as meiotic silencing by unpaired DNA (MSUD). MSUD utilizes common RNA interference (RNAi) factors to silence target mRNAs. Here, we report that Neurospora CAR-1 and CGH-1, homologs of two Caenorhabditis elegans RNA granule components, are involved in MSUD. These fungal proteins are found in the perinuclear region and P-bodies, much like their worm counterparts. They interact with components of the meiotic s ...[more]

PMID: 34568932

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