Relief of ParB autoinhibition by parS DNA catalysis and recycling of ParB by CTP hydrolysis promote bacterial centromere assembly.
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ABSTRACT:
SUBMITTER: Antar H
PROVIDER: S-EPMC8494293 | biostudies-literature | 2021 Oct
REPOSITORIES: biostudies-literature
Publications
Relief of ParB autoinhibition by <i>parS</i> DNA catalysis and recycling of ParB by CTP hydrolysis promote bacterial centromere assembly.
Antar Hammam H Soh Young-Min YM Zamuner Stefano S Bock Florian P FP Anchimiuk Anna A Rios Paolo De Los PL Gruber Stephan S
Science advances 20211006 41
Three-component ParABS systems are widely distributed factors for plasmid partitioning and chromosome segregation in bacteria. ParB acts as adaptor protein between the 16–base pair centromeric <i>parS</i> DNA sequences and the DNA segregation proteins ParA and Smc (structural maintenance of chromosomes). Upon cytidine triphosphate (CTP) and <i>parS</i> DNA binding, ParB dimers form DNA clamps that spread onto <i>parS</i>-flanking DNA by sliding, thus assembling the so-called partition complex. W ...[more]
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