Morphological diversity of single neurons in molecularly defined cell types.

ABSTRACT: Dendritic and axonal morphology reflects the input and output of neurons and is a defining feature of neuronal types^1,2, yet our knowledge of its diversity remains limited. Here, to systematically examine complete single-neuron morphologies on a brain-wide scale, we established a pipeline encompassing sparse labelling, whole-brain imaging, reconstruction, registration and analysis. We fully reconstructed 1,741 neurons from cortex, claustrum, thalamus, striatum and other brain regions in mice. We identified 11 major projection neuron types with distinct morphological features and corresponding transcriptomic identities. Extensive projectional diversity was found within each of these major types, on the basis of which some types were clustered into more refined subtypes. This dive

SUBMITTER: Peng H 

PROVIDER: S-EPMC8494643 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature