Novel Simple Conjugation Chemistries for Decoration of GMMA with Heterologous Antigens.

ABSTRACT: Outer Membrane Vesicles (OMV) constitute a promising platform for the development of efficient vaccines. OMV can be decorated with heterologous antigens (proteins or polysaccharides), becoming attractive novel carriers for the development of multicomponent vaccines. Chemical conjugation represents a tool for linking antigens, also from phylogenetically distant pathogens, to OMV. Here we develop two simple and widely applicable conjugation chemistries targeting proteins or lipopolysaccharides on the surface of Generalized Modules for Membrane Antigens (GMMA), OMV spontaneously released from Gram-negative bacteria mutated to increase vesicle yield and reduce potential reactogenicity. A Design of Experiment approach was used to identify optimal conditions for GMMA activation before conjugatio

SUBMITTER: Di Benedetto R 

PROVIDER: S-EPMC8508390 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature