MicroRNA-501-3p inhibits the proliferation of kidney cancer cells by targeting WTAP.

ABSTRACT:

Background

Emerging evidence suggests that miR-501-3p plays an important role in the pathogenesis and progression of various carcinomas. However, its role and underlying mechanisms in renal cell carcinoma (RCC) remain to be elucidated.

Methods

Quantitative RT-PCR, western blot, and bioinformatics methods were used to evaluate the expression of miR-501-3p and Wilms' tumor 1-associating protein (WTAP) in RCC cell lines and clinical tissues. The effects of miR-501-3p on the proliferation of RCC cells were investigated using flow cytometric, colony formation, and CCK8 assays. The target gene of miR-501-3p was confirmed by western blotting, qRT-PCR, and dual-luciferase reporter assays. The levels of RNA methylation with N6-methyladenosine (m⁶ A) following miR-501-3p ov

SUBMITTER: He L 

PROVIDER: S-EPMC8525086 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature