Differential activation of Ca²⁺ influx channels modulate stem cell potency, their proliferation/viability and tissue regeneration.

ABSTRACT: Stem cells have indefinite self-renewable capability; however, factors that modulate their pluripotency/function are not fully identified. Here we show that store-dependent Ca²⁺ entry is essential for modulating the function of bone marrow-derived mesenchymal stem cells (MSCs). Increasing external Ca²⁺ modulated cell cycle progression that was critical for MSCs survival. Additionally, Ca²⁺ was critical for stem proliferation, its differentiation, and maintaining stem cell potential. Ca²⁺ channel characterization, including gene silencing, showed two distinct Ca²⁺ entry channels (through Orai1/TRPC1 or via Orai3) that differentially regulate the proliferation and viability of MSCs. Importantly, NFκB translocation, but not JNK/ERK into t

SUBMITTER: Ahamad N 

PROVIDER: S-EPMC8528841 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature