GOBP1 from the Variegated Cutworm Peridroma saucia (Hubner) (Lepidoptera: Noctuidae) Displays High Binding Affinities to the Behavioral Attractant (Z)-3-Hexenyl acetate.

ABSTRACT: The variegated cutworm Peridroma saucia (Hübner) is a worldwide pest that causes serious damage to many crops. To recognize sex pheromones and host plant volatiles, insects depend on olfactory chemoreception involving general odorant-binding proteins (GOBPs). In this study, PsauGOBP1 was cloned from the adult antennae of P. saucia. RT-qPCR and Western-blot analysis showed that PsauGOBP1 was specifically and equally expressed in the adult antennae of both females and males. Fluorescence competitive-binding assays with sex pheromones and host plant volatiles demonstrated that PsauGOBP1 bound to six host plant volatiles: (Z)-3-hexenyl acetate (K_D = 4.0 ± 0.1 μM), citral (K_D = 5.6 ± 0.4 μM), farnesol (K_D = 6.4 ± 0.6 μM), nonanal (K

SUBMITTER: Sun YL 

PROVIDER: S-EPMC8540349 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature