Identification of Non-Canonical Translation Products in C. elegans Using Tandem Mass Spectrometry.

ABSTRACT: Transcriptome and ribosome sequencing have revealed the existence of many non-canonical transcripts, mainly containing splice variants, ncRNA, sORFs and altORFs. However, identification and characterization of products that may be translated out of these remains a challenge. Addressing this, we here report on 552 non-canonical proteins and splice variants in the model organism C. elegans using tandem mass spectrometry. Aided by sequencing-based prediction, we generated a custom proteome database tailored to search for non-canonical translation products of C. elegans. Using this database, we mined available mass spectrometric resources of C. elegans, from which 51 novel, non-canonical proteins could be identified. Furthermore, we utilized diverse proteomic

SUBMITTER: Parmar BS 

PROVIDER: S-EPMC8575065 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature