Posttranscriptional control of PLOD1 in adipose-derived stem cells regulates scar formation through altering macrophage polarization.

ABSTRACT:

Background

The level of cutaneous scar formation is a critical parameter to determine the success of skin wound healing. Adipose-derived mesenchymal stem cells (AMSCs) have been applied to improve treatment of cutaneous injury with the purpose of reducing scar formation.

Methods

The levels of procollagen-lysine 1,2-oxoglutarate 5-dioxygenase 1 (PLOD1) were assessed at scar sites. Then, PLOD1 in AMSCs was depleted by either expression of a PLOD1-specific short-hair interfering RNA (shPLOD1) or by expression of microRNA-449 (miR-449) that targets and suppresses protein translation of PLOD1 through 3 prime untranslated region (3'-UTR) interfering. For induction of skin injury, a blade cut of 1.5-cm long and 2-mm thick was made on the middle back of the mice

SUBMITTER: Xu M 

PROVIDER: S-EPMC8576667 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature