Dataset Information

The transposable element-rich genome of the cereal pest Sitophilus oryzae.

ABSTRACT:

Background

The rice weevil Sitophilus oryzae is one of the most important agricultural pests, causing extensive damage to cereal in fields and to stored grains. S. oryzae has an intracellular symbiotic relationship (endosymbiosis) with the Gram-negative bacterium Sodalis pierantonius and is a valuable model to decipher host-symbiont molecular interactions.

Results

We sequenced the Sitophilus oryzae genome using a combination of short and long reads to produce the best assembly for a Curculionidae species to date. We show that S. oryzae has undergone successive bursts of transposable element (TE) amplification, representing 72% of the genome. In addition, we show that many TE families are transcriptionally active, and changes in their expression are associated with insect endosymbiotic state. S. oryzae has undergone a high gene expansion rate, when compared to other beetles. Reconstruction of host-symbiont metabolic networks revealed that, despite its recent association with cereal weevils (30 kyear), S. pierantonius relies on the host for several amino acids and nucleotides to survive and to produce vitamins and essential amino acids required for insect development and cuticle biosynthesis.

Conclusions

Here we present the genome of an agricultural pest beetle, which may act as a foundation for pest control. In addition, S. oryzae may be a useful model for endosymbiosis, and studying TE evolution and regulation, along with the impact of TEs on eukaryotic genomes.

SUBMITTER: Parisot N

PROVIDER: S-EPMC8576890 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

The transposable element-rich genome of the cereal pest Sitophilus oryzae.

Parisot Nicolas N Vargas-Chávez Carlos C Goubert Clément C Baa-Puyoulet Patrice P Balmand Séverine S Beranger Louis L Blanc Caroline C Bonnamour Aymeric A Boulesteix Matthieu M Burlet Nelly N Calevro Federica F Callaerts Patrick P Chancy Théo T Charles Hubert H Colella Stefano S Da Silva Barbosa André A Dell'Aglio Elisa E Di Genova Alex A Febvay Gérard G Gabaldón Toni T Galvão Ferrarini Mariana M Gerber Alexandra A Gillet Benjamin B Hubley Robert R Hughes Sandrine S Jacquin-Joly Emmanuelle E Maire Justin J Marcet-Houben Marina M Masson Florent F Meslin Camille C Montagné Nicolas N Moya Andrés A Ribeiro de Vasconcelos Ana Tereza AT Richard Gautier G Rosen Jeb J Sagot Marie-France MF Smit Arian F A AFA Storer Jessica M JM Vincent-Monegat Carole C Vallier Agnès A Vigneron Aurélien A Zaidman-Rémy Anna A Zamoum Waël W Vieira Cristina C Rebollo Rita R Latorre Amparo A Heddi Abdelaziz A

BMC biology 20211109 1

<h4>Background</h4>The rice weevil Sitophilus oryzae is one of the most important agricultural pests, causing extensive damage to cereal in fields and to stored grains. S. oryzae has an intracellular symbiotic relationship (endosymbiosis) with the Gram-negative bacterium Sodalis pierantonius and is a valuable model to decipher host-symbiont molecular interactions.<h4>Results</h4>We sequenced the Sitophilus oryzae genome using a combination of short and long reads to produce the best assembly for ...[more]

PMID: 34749730

Similar Datasets

Project description:BackgroundInsects thriving on nutritionally poor habitats have integrated mutualistic intracellular symbiotic bacteria (endosymbionts) in a bacteria-bearing tissue (the bacteriome) that isolates the endosymbionts and protects them against a host systemic immune response. Whilst the metabolic and physiological features of long-term insect associations have been investigated in detail over the past decades, cellular and immune regulations that determine the host response to endosymbionts and pathogens have attracted interest more recently.ResultsTo investigate bacteriome cellular specificities and weevil immune responses to bacteria, we have constructed and sequenced 7 cDNA libraries from Sitophilus oryzae whole larvae and bacteriomes. Bioinformatic analysis of 26,886 ESTs led to the generation of 8,941 weevil unigenes. Based on in silico analysis and on the examination of genes involved in the cellular pathways of potential interest to intracellular symbiosis (i.e. cell growth and apoptosis, autophagy, immunity), we have selected and analyzed 29 genes using qRT-PCR, taking into consideration bacteriome specificity and symbiosis impact on the host response to pathogens. We show that the bacteriome tissue accumulates transcripts from genes involved in cellular development and survival, such as the apoptotic inhibitors iap2 and iap3, and endosomal fusion and trafficking, such as Rab7, Hrs, and SNARE. As regards our investigation into immunity, we first strengthen the bacteriome immunomodulation previously reported in S. zeamais. We show that the sarcotoxin, the c-type lysozyme, and the wpgrp2 genes are downregulated in the S. oryzae bacteriome, when compared to aposymbiotic insects and insects challenged with E. coli. Secondly, transcript level comparison between symbiotic and aposymbiotic larvae provides evidence that the immune systemic response to pathogens is decreased in symbiotic insects, as shown by the relatively high expression of wpgrp2, wpgrp3, coleoptericin-B, diptericin, and sarcotoxin genes in aposymbiotic insects.ConclusionsLibrary sequencing significantly increased the number of unigenes, allowing for improved functional and genetic investigations in the cereal weevil S. oryzae. Transcriptomic analyses support selective and local immune gene expression in the bacteriome tissue and uncover cellular pathways that are of potential interest to bacteriocyte survival and homeostasis. Bacterial challenge experiments have revealed that the systemic immune response would be less induced in a symbiotic insect, thus highlighting new perspectives on host immunity in long-term invertebrate co-evolutionary associations.

Dataset Information

The transposable element-rich genome of the cereal pest Sitophilus oryzae.

Background

Results

Conclusions

Publications

The transposable element-rich genome of the cereal pest Sitophilus oryzae.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets