Manufacturing T cells in hollow fiber membrane bioreactors changes their programming and enhances their potency.

ABSTRACT: Engineered T cell therapies have revolutionized modern oncology, however processes for manufacturing T cell therapies vary and the impact of manufacturing processes On the cell product is poorly understood. Herein, we have used a commercially available hollow fiber membrane bioreactor (HFMBR) operated in a novel mode to demonstrate that T cells can be engineered with lentiviruses, grown to very high densities, and washed and harvested in a single, small volume bioreactor that is readily amenable to automation. Manufacturing within the HFMBR dramatically changed the programming of the T cells and yielded a product with greater therapeutic potency than T cells produced using the standard manual method. This change in programming was associated with increased resistance to cryopreservation, w

SUBMITTER: Yoo SM 

PROVIDER: S-EPMC8583081 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature