Biochemical analysis of cleavage and ligation activities of the pistol ribozyme from Paenibacillus polymyxa.

ABSTRACT: Nine distinct classes of self-cleaving ribozymes are known to date, of which the pistol ribozyme class was discovered only 5 years ago. Self-cleaving ribozymes are able to cleave their own phosphodiester backbone at a specific site with rates much higher than those of spontaneous RNA degradation. Our study focuses on a bioinformatically predicted pistol ribozyme from the bacterium Paenibacillus polymyxa. We provide a biochemical characterization of this ribozyme, which includes an investigation of the effect of various metal ions on ribozyme cleavage and a kinetic analysis of ribozyme activity under increasing Mg²⁺ concentrations and pH. Based on the obtained results, we discuss a possible catalytic role of divalent metal ions. Moreover, we investigated the ligation activ

SUBMITTER: Lihanova Y 

PROVIDER: S-EPMC8583172 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature