Immunomodulation of endothelial cells induced by macrolide therapy in a model of septic stimulation.

ABSTRACT:

Objectives

Sepsis is defined as the host's inflammatory response to a life-threatening infection. The endothelium is implicated in immunoregulation during sepsis. Macrolides have been proposed to display immunomodulatory properties. The goal of this study was to analyze whether macrolides can exert immunomodulation of endothelial cells (ECs) in an experimental model of sepsis.

Methods

Human ECs were stimulated by proinflammatory cytokines and lipopolysaccharide before exposure to macrolides. ECs phenotypes were analyzed by flow cytometry. Cocultures of ECs and peripheral blood mononuclear cells (PBMCs) were performed to study the ECs ability to alter T-cell viability and differentiation in the presence of macrolides. Soluble factor production was assessed.

Results

ECs

SUBMITTER: Pons S 

PROVIDER: S-EPMC8589380 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature