Dataset Information
Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2.
Ontology highlight
ABSTRACT: Background
Current assays for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus isolation, purification and removing inhibitors. To address this limitation, we propose a simple method for testing RNA from nasopharyngeal swab samples that bypasses the RNA purification step.Methods
In the current project, we have described two extraction-free reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for the detection of SARS-CoV-2 by using E gene and RdRp gene as the targets.Results
Here, results showed that reverse transcription loop-mediated isothermal amplification assays with 88.4% sensitive (95% CI: 74.9-96.1%) and 67.4% sensitive (95% CI: 51.5-80.9%) for E gene and RdRp gene, respectively.Conclusion
Without the need of RNA purification, our developed RT-LAMP assays for direct detection of SARS-CoV-2 from nasopharyngeal swab samples could be turned into alternatives to qRT-PCR for rapid screening.
SUBMITTER: Lai MY
PROVIDER: S-EPMC8595270 | biostudies-literature | 2021 Nov
REPOSITORIES: biostudies-literature
Publications
Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2.
BMC infectious diseases 20211117 1
<h4>Background</h4>Current assays for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus isolation, purification and removing inhibitors. To address this limitation, we propose a simple method for testing RNA from nasopharyngeal swab samples that bypasses the RNA purification step.<h4>Methods</h4>In the current project, we have described two extraction-free reverse transcription loop-mediated isothermal ...[more]