Location-Dependent Lanthanide Selectivity Engineered into Structurally Characterized Designed Coiled Coils.

ABSTRACT: Herein we report unprecedented location-dependent, size-selective binding to designed lanthanide (Ln³⁺ ) sites within miniature protein coiled coil scaffolds. Not only do these engineered sites display unusual Ln³⁺ selectivity for moderately large Ln³⁺ ions (Nd to Tb), for the first time we demonstrate that selectivity can be location-dependent and can be programmed into the sequence. A 1 nm linear translation of the binding site towards the N-terminus can convert a selective site into a highly promiscuous one. An X-ray crystal structure, the first of a lanthanide binding site within a coiled coil to be reported, coupled with CD studies, reveal the existence of an optimal radius that likely stems from the structural constraints of the coiled coil scaffold.

SUBMITTER: Slope LN 

PROVIDER: S-EPMC8597134 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature