Inulin-grown Faecalibacterium prausnitzii cross-feeds fructose to the human intestinal epithelium.

ABSTRACT: Many chronic diseases are associated with decreased abundance of the gut commensal Faecalibacterium prausnitzii. This strict anaerobe can grow on dietary fibers, e.g., prebiotics, and produce high levels of butyrate, often associated to epithelial metabolism and health. However, little is known about other F. prausnitzii metabolites that may affect the colonic epithelium. Here, we analyzed prebiotic cross-feeding between F. prausnitzii and intestinal epithelial (Caco-2) cells in a "Human-oxygen Bacteria-anaerobic" coculture system. Inulin-grown F. prausnitzii enhanced Caco-2 viability and suppressed inflammation- and oxidative stress-marker expression. Inulin-grown F. prausnitzii produced excess butyrate and fructose, but only fructose efficiently promote

SUBMITTER: Fagundes RR 

PROVIDER: S-EPMC8604389 | biostudies-literature | 2021 Jan-Dec

REPOSITORIES: biostudies-literature