Transcriptional landscape of highly lignified poplar stems at single-cell resolution.

ABSTRACT:

Background

Plant secondary growth depends on the activity of the vascular cambium, which produces xylem and phloem. Wood derived from xylem is the most abundant form of biomass globally and has played key socio-economic and subsistence roles throughout human history. However, despite intensive study of vascular development, the full diversity of cell types and the gene networks engaged are still poorly understood.

Results

Here, we have applied an optimized protoplast isolation protocol and RNA sequencing to characterize the high-resolution single-cell transcriptional landscape of highly lignified poplar stems. We identify 20 putative cell clusters with a series of novel cluster-specific marker genes and find that these cells are highly heterogeneous based on the transcriptom

SUBMITTER: Chen Y 

PROVIDER: S-EPMC8607660 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature